Publications

The phytochemical constituents were identified, and the antioxidant, antiinflammatory, antiacetylcholinesterase (AChE), cytotoxic, and anti-haemolytic properties were assessed for the leaves and stem extracts of Lebanese rose geranium extracted by four solvents with different polarity (ethanol, water, ethyl acetate and chloroform). The highest amounts of total phenolics (237 GAE mg/g), total flavonoids (356 RE mg/g) and condensed tannins content (441 LE mg/g) were detected in the ethanolic leaves extracts. The antioxidant activity was estimated by four methods, namely DPPH radical scavenging, hydrogen peroxide scavenging, reducing power and β-carotene bleaching assays and the ethanolic leaves extract exhibited the superior antioxidant potential. On the other hand, the chloroform extracts revealed good anti-inflammatory activity. The ethanol and ethyl acetate leaves and stem extracts demonstrated the highest anti-AChE activity with 80% inhibition achieved at 5 mg/mL. The cytotoxic activity of the extracts was evaluated against HCT 116 colon cancer cells, with the chloroform leaves extract being the most potent with an IC50 of 0.4 mg/mL. The toxicity of the extracts was assessed by testing its ability to induce haemolysis of red blood cells, and the water leaves extract was the least toxic.