Validated Kinetic Spectrophotometric Determination of Pitavastatin Calcium Using Acidic Permanganate Oxidation
Objective: Development and validation of a sensitive, indirect spectrophotometric kinetic method, based on oxidation-reduction reaction, using
potassium permanganate, for the quantitative assay of pitavastatin calcium, a cardiovascular drug used for the treatment of hyperlipidemia.
Methods: The developed spectrophotometric kinetic method is based on the ability of potassium permanganate
Results: During the course of the reaction, the absorbance values, at 525 nm, related to KMnO
to oxidize Pitavastatin, where, the
drug solution is treated with a fixed concentration of permanganate in acidic medium, and after a specified time, the unreacted permanganate is
measured at 525 nm. All variables affecting the color development have been investigated and the conditions were optimized. Different kinetic
methods, including initial rate, rate constant, fixed time and fixed concentration, were applied for the determination Pitavastatin.
Conclusion: The developed indirect spectrophotometric kinetic method, using the fixed time method, was used for the determination of
Pitavastatin in pharmaceutical tablets. This method was simple, accurate and easy to apply for routine assay and in quality control laboratories.
decreased linearly with increasing the
concentration of the drug. The reaction rate obeyed was found to be pseudo-first-order and the kinetic method used was the fixed-time method. The
assay of PITA in the concentration range of 16-80 μg/ml, using the fixed time method was successfully determined with a correlation coefficient
value of 0.9999. The applicability of the developed method was also demonstrated by the determination of pitavastatin in its pure form and in its
pharmaceutical formulation, where, the effect of excipients has also been studied and found to have no effect.
Keywords: Pitavastatin Calcium, Potassium Permanganate, Oxidation, Kinetic methods and Spectrophotometry
Marwa Khaled Al Jamal
International Journal of Pharmacy and Pharmaceutical Sciences,DOI: 10.22159/ijpps.2020v12i3.35888, ISSN: 2656-0097 , Volume: 2, Issue: 3, Pages Range: 28-33,