Phytochemical and anti-neuropathic investigations of Crocus sativus via alleviating inflammation, oxidative stress and pancreatic beta-cells regeneration
Objectives: The aim of this study is to investigate the phytochemical and the long-term anti-neuropathic potentials of Crocus
sativus cultivated in the University botanical garden, and explore its most bioactive compounds and their underlying
mechanisms of action.
Methods: Phytochemical analysis and bio-guided isolation-procedures including RP-HPLC and 1H and 13C NMR utilizing
biological models of diabetes, inflammation, and diabetic-neuropathy were used. Cultivated saffron (S-RCED) and Spanishsaffron
stigma (S-SP) alone or in combination with Camellia sinus (CS) were investigated.
Results: The RP-HPLC analyses showed the presence of picrocrocin, crocin I, crocin II, crocin I’, crocin II’, and safranal (SAF)
in both S-SP and S-RCED extracts with higher-concentrations. It had been shown that SAF was the most bioactive-compound
in Crocus sativus. Both S-SP and S-RCED possessed significant (P ˂ 0.05) anti-diabetic activities in acute (6 h), subchronic (8
d) and chronic (8 weeks) models. S-RCED had been proven with more hypoglycemic potentials when compared to S-SP and
SAF. S-SP, S-RCED, and SAF produced significant anti-inflammatory and anti-nociceptive activities against carrageenaninduced
inflammatory, hyperalgesic and tactile diabetic-neuropathy models, respectively. S-SP, S-RCED, and SAF elevated
serum catalase, reduced glutathione, and insulin serum levels, ameliorated lipid peroxidation and HbA1c levels, and
histopathologically regenerated the pancreatic beta-cells. Combinations with CS showed more significant efficacy than the
Conclusion: The oxidative stress reduction, insulin secretagogue, and pancreatic beta-cells regeneration potentials might be
responsible for the mechanism underlying the anti-diabetic, anti-inflammatory and anti-diabetic neuropathy activities. Thus, the
cultivated Crocus sativus might be clinically useful for protecting against many serious-disorders.
Maha A. Aboul-Ela
Chinese Herbal Medicines,DOI: https://doi.org/10.1016/j.chmed.2019.07.004, ISSN: 2589-3610, Volume: E2, Issue: E1, Pages Range: 1-16,