Infrared-assisted extraction and HPLC-analysis of Prunus Armeniaca L. pomace and detoxified-kernel and their antidiabetic effects
Introduction - Prunus armeniaca L. (P. armeniaca) is one of the medicinal plants with high safety-profile.
Objectives - The aim of this work was to make an Infrared-assisted extraction of P. armeniaca fruit (pomace) and kernel, and analyze them utilizing RP-HPLC aided method.
Methods- Infrared-assisted extraction (IR-AE) is a novel-technique aiming at increasing the extraction-efficiency. The antidiabetic-potentials of the P. armeniaca pomace (AP) and the detoxified-kernel (DKAP) were monitored exploring their possible hypoglycemic-mechanisms. Acute (6h), subchronic (8 days) and long-term (8 weeks) assessment of DM utilizing glucometers and HbA1c methods have been applied.
Results- Serum-insulin levels, the inhibitory effects on alpha-glucosidase, serum-catalase (CAT) and lipid-peroxidation (LPO) levels have been also monitored. AP has shown to be rich in polyphenolics like trans-lutein (14.1%), trans-zeaxanthin (10.5%), trans- ß -cryptoxanthin (11.6%),13, cis-ß-carotene (6.5%), trans 9, cis-ß-carotene (18.4%), and ß-carotene (21.5%). KAP was found to be rich in amygdaline (16.1%), which caused high mortality-rate (50.1%), while after detoxification (amygdaline, 1.4%) has shown lower mortality-rate (9.1%). AP has shown significant (p≤0.05, n=7/group) antidiabetic-activity more prominent than DKAP acutely, subchronically and on longer-terms. IR-AE extracts have shown more efficient acute and subchronic BGL reduction than conventional extraction method, which might be attributed to IR-AE superiority in extraction of active ingredients. AP has shown more-significant and dose-dependent increase in serum-insulin, CAT-levels and body-weights more prominent than those of DKAP. Alpha glucosidase and LPO levels were inhibited with AP-groups more-significantly.
Conclusion - In comparison to conventional-methods, IR-AE has shown to be an efficient and time-conserving novel extraction method. The antidiabetic-potentials of pomace and detoxified-kernels of P. armeniaca were probably mediated via the attenuation of glucose-provoked oxidative-stress, the inhibition of alpha-glucosidase and the marked insulin-secretagogue effect.
Karim M Raafat, Nada El-Darra, Fatima Saleh, Hiba N. Rajha, Richard G. Maroun
Journal of Phytochemical Analysis,DOI: 10.1002/pca.2723, ISSN: 1099-1565, Volume: 10.1002/pca.2723, Issue: 10.1002/pca.2723, Pages Range: 1-12